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  Digital Catalogue for Technology and Products Development


   Technology and Product Development

    Basic Information

Technology developed: PROCESS FOR PRODUCING RECOMBINANT SERRATIOPEPTIDASE (TP19762341296)
Category: Nil
Details of Inventor(s):
Inventor Institution/Organization/Company Department Designation
Prof. Tapan Chaudhari IIT Delhi Kusuma School Of Biological Sciences Professor
Technical Application Area: Healthcare
If 'Other', please specify:
Please give more details of new technical application area:
Organization(s):
Indian Institute of Technology (IIT) Delhi
Affiliated Ministry: Institution Funding (Self Supported)
Type of technology development: Indigenous
Does the technology help in replacing any import items currently
procured from outside India?
Does the technology have export potential? 1
Category of Technology developed: Immediate Deployment
Stage of Development: Lab-scale
Please describe in detail including the TRL Level:
TRL4

    Abstract:

Applications: Serratiopeptidase, a proteolytic enzyme, has many clinical and biotechnological applications: • Active ingredient in various drug combinations due to its potent anti-inflammatory, analgesic, anti-edemic effects • Used for the treatment of chronic sinusitis, carpal tunnel syndrome, sprains, torn ligaments, and post-operative inflammation • Health supplement for many conditions to prevent morbidity (e.g., cardiovascular morbidity) • Additive in detergents, leather and paper industry, food processing • Activity against biofilm formation and amyloid degradation Serratiopeptidase is produced using wild and mutant strains of enterobacterium Serratia marcescens ATCC 21074. This approach is source organism dependent and has a narrow scope of optimization, hence limiting the yield. Moreover, the organism is an opportunistic pathogenic and is associated with a variety of infections. Recombinant expression of serratiopeptidase is a viable solution but has various difficulties in terms of poor expression levels, stability issues and inactive protein due to inclusion bodies. Also, heterologous expression of proteases causes critical cellular stress leading to toxicity and death of host cells. Purifying proteins from inclusion bodies is a tedious job and adds to the cost. The invention relates to an optimized recombinant overexpression methodology for high-level expression of serratiopeptidase in E. coli. The enzyme is expressed in its non-toxic, inactive, zymogenic version which is segregated into the inclusion bodies resulting in proper cell growth and survival of the recombinant host cells. The protocol is optimized with respect to various physicochemical parameters leading to significant enhancement in yields. The inclusion bodies are solubilized and the protein is processed and purified to obtain the mature active enzyme.
Advantages: 1. By using E. coli as the expression system, the health hazards associated with native and mutant strains of Serratia marcescens are overcome. 2. E. coli is the most convenient and well studied host system as compared to the other host systems. The production is simple, less time consuming and cost-effective; Simple down-stream processing which overcomes the problems associated with other systems 3. The protein is expressed in its non-toxic, inactive, zymogenic version which is segregated into inclusion bodies resulting in proper cell growth and survival of the recombinant host E. coli cells. The inclusion bodies are then solubilized and protein is processed and purified to obtain the mature active enzyme 4. The enzyme is obtained at high yields and activity levels 5. The product is highly similar and bioequivalent to the commercial serratiopeptidase

    Technology Inputs:

Imported Equipment/Spare Parts:
Equipment/Spare Parts Year ITC-HS Code
NA
Indigenous Equipment/Spare Parts:
Equipment/Spare Parts Year ITC-HS Code
NA
Imported Raw Materials:
Raw Materials Year ITC-HS Code
NA
Indigenous Raw Materials:
Raw Materials Year ITC-HS Code
NA
Existing R&D Facilities used:
Facilities Year ITC-HS Code
NA

    R&D Investment:

R&D investment (Rs. in Lakhs):                    
Indian Source (Rs.) Foreign Source (Rs.) Year
0 0 NA

   Patents & Publications:

Patents:
Filed Patents (No.) Granted Patents (No.) Year
0 0 NA
Publications:
Submitted (No.) Published (No.) Year
0 0 NA

    Commercialization Potential:

Who are the Potential Licensees?
What commercially available products address
the same problem?
Company Product Problem Addressed
Would you like to develop this invention further with
corporate research support?
Yes
Would you be interested in participating in cluster based
programs for commercialization research or business
planning for your invention?
Yes
      Submitted by: Anil Wali Date of Submission: 5-8-2020



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