Indian Science Technology and Engineering facilities Map
Supplier Map
Service Map


Patent Details

Indian Institute of Technology (IIT) Roorkee 
A Novel phytase and process for enhancing phytase titer 
Sanjoy Ghosh and Richa Rani  
Indian Institute of Technology (IIT) Roorkee 
Application type:
Applied - 2010-04-29 
Application Number:
Applied at:
Entered by:
Indian Institute of Technology (IIT) Roorkee 
This invention relates to a phytase of low Michaelis-Menten constant (Km) value from a fungal strain, Rhizopus Oryzae, wherein the phytase has the following physiochemical properties: low Michaelis-Menten constant (Km) of 2.42 × 10-4 mM; Molecular weight of 34 kDa; optimum temperature of 45ºC; optimum pH of 1.5 and 5.5; temperature stability up to 100 ºC; pH stability: stable in the range of pH 1.0 to 10.0, excellent enzyme activity at a physiological temperature (39 ºC), Pepsin resistant, low metal ions sensitivity, wide range of substrate specificity; acting on phytate, p-Nitro Phenyl Phosphate (PNPP), Nicotinamide adenine dinucleotide phosphate (NADP), Sodium-1-naphthyl phosphate (SNP), Fructose-1, 6-biphosphate (FBP), Ribose-5-phosphate (RBP), Glucose-1- phosphate (G-1-P), Phosphoenol pyruvic acid (PEP), Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), and Adenosine Triphosphtae (ATP) as a substrate and a process for producing a novel phytase of low Michaelis-Menten constant (Km) value from a fungi by strain improvement process comprising the steps of, a. culturing and maintain the fungal strain on potato dextrose agar pertiplates and slants; b. harvesting viable spores from 6 day old fully sporulated slants by washing with tween-80 containing DW; c. stressing the viable spores of fungal strain by exposing the cells to heat and cold stress, subsequently; d. adjusting the spore suspension to 1×106 cfu (colony forming units on PDA plates) per ml for inoculation; e. inoculation one milliliter spore suspension of step d. to the solid state fermentation medium in a flask; f. subjecting the flask to stationary culture at a temperature of 30 ºC for 72 hrs for phytase production; g. adding 0.1% of tween-80 containing distilled water to the flask with shaking at 200 rpm at temperature of 30 ºC for one hour; h. separating the crude extracts containing the phytase by centrifugation at 12, 000 rpm for 10 min to obtain the supernatant containing crude phytase, i. resulting in enhance enzyme titer up to 60%.  

I-Mitra(आई-मित्र) Welcomes You..
It has always been the basic tenet of the Government of India, in generously funding R&D efforts at academic institutions over the years, that facilities established through such support be made available to those needing them and qualified to make use of them for their own research work

However, this was never easy or straightforward for, among other reasons, there was no ready source of information of what facility was available and where. Thanks to the Web, it is much easier today to have a national and regional “inventory of resources”, so as to match users with the resources they need, and to do all this in an efficient and transparent manner.

This can lead to a leap in R&D productivity and greatly enhance the effectiveness of public investment. This is the motivation behind I-STEM.
read less <<
Visitor Hit Counter
Hosted at Indian Institute of Science
Copyright © 2020 I-STEM. All rights reserved.
Audited by: STQC Bengaluru.