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Applicant:
Indian Institute of Technology (IIT) Roorkee 
Title:
Asparaginase and its variants 
Inventor(s):
Naveen Kumar Navani*, and Tamoghna Ghosh 
Application type:
Applied - 2018-05-03 
Application Number:
201811019299 
Applied at:
India 
Entered by:
Indian Institute of Technology (IIT) Roorkee 
Abstract:
The present invention is to provide an effective mutant of bacterial enzyme from probiotic source i.e. Lactobacillus brevis NKN55 with improved efficacy and thermostability useful for therapeutics and food industry. L-asparaginase increased enzymatic reaction optimum temperatures with no significant alteration to substrate affinity and reaction velocity. The substrate affinity of the mutant enzyme was calculated by Michaelis-Menten equation. It also provide therapeutically effective and immunologically-distinct, alternative and stable mutated form of L-asparaginase enzyme from a food grade probiotic lactic acid bacterium and also by using site specific mutagenesis. 

 
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