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Indian Institute of Technology (IIT) Roorkee 
Fluorescence based cost-effective rapid diagnostic kit for detection of cancer biomarkers 
P. Gopinath, Ashish and Rangadhar Pradha 
Indian Institute of Technology (IIT) Roorkee 
Application type:
Applied - 2020-03-09 
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Indian Institute of Technology (IIT) Roorkee 
The present invention relates to a fluorescence based rapid diagnostic kit for highly sensitive detection of cancer cell-specific biomarkers utilizing biofunctionalized graphene quantum dots as donor and gold nanoparticles as acceptor in a fluorescence based assay. The invention discloses a kit for detection of cancer biomarkers, more specifically, of small cell lung cancer biomarker by using anti-neuron specific enolase antibody conjugated to amine functionalized nitrogen doped graphene quantum dots. The invention is directed to methods and compositions for making the diagnostic kit. The novel process discloses one-pot, low temperature, and the bottom-up hydrothermal route for the synthesis of in-situ amine-functionalized and nitrogen-doped graphene quantum dots. 

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