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Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
Process for Extraction of Superior Quality Plasmid DNA  
Ranga Udaykumar 
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
Application type:
Granted - 2005-05-24 
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Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
An improved process for the extraction of superior quality plasmid DNA, said process comprising the steps of: a. harvesting bacterial cells by centrifugation, b. suspending the harvested cells by adding solution II such as here described, d. neutralising the lysed calls by adding solution III such as here in described, e. centrifuging the neutralized cells and thereby precipitating cell debris and proteins and to obtain a supernatant, f. adding NaCl stock into the supernatant to increase concentration of Na+ ions in the supernatant, g. adding a silica suspension such as herein described to the supernatant to obtain a silica solution, h. centrifuging the silica solution of step bound to silica pellets, i. washing the silica pellets with a buffer to remove impurities and j. eluting the bound plasmid DNA with distilled water or low salt buffer and thereby obtaining the superior quality plasmid DNA. 

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