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Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
Monoclonal Antibodies Against NPM1 and Acetylated NPM1, Methods and Kit Thereof 
Tapas Kumar Kundu, Parijat Senapati, Gopinath Kodaganur Srinivasachar, Deepthi Sudarshan, Manjula Das, Smitha Pazhoor Kumaran, Manjunath Shivasangappa Devaraman, Ajithkumar Sumitrappa 
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
Application type:
Granted - 2019-03-21 
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Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bengaluru 
NPM1 is a multifunctional nucleolar protein, which dynamically shuttles from nucleolus to nucleus and cytoplasm depending on the cellular signals. It is a histone chaperone and induces chromatin mediated transcription. NPM1 gets specifically acetylated by the Lysine Acetyl Transferase, p300. Acetylation of NPM1 enhances its chaperone activity and is essential for transcriptional coactivation property. NPM1 gets overexpressed and hyperacetylated in oral cancer. We have identified few sites of acetylation in NPM1 by Mass Spectrometric analysis of the in vivo protein. Based on this data acetylated peptide has been designed and monoclonal antibodies have been raised. Several positive clones have been characterized. A few site specific antibodies could be generated along with monoclonal antibodies against unmodified NPM1 also. The monoclonal antibody against unmodified NPM1 could recognize the protein in the cell lines, human tissue and also the recombinant forms. The unmodified protein is predominantly localized in the nucleolus and the acetylated NPM1 is predominantly found in the nucleoplasm, but some site specific antibodies could recognize the modified protein in the nucleolus. These antibodies will not only be useful as academic reagents to understand the dynamic role of NPM1 but will also serve as diagnostic reagents for cancer, especially oral cancer. 

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