Indian Science Technology and Engineering facilities Map
 
Supplier Map
Service Map

Publications

Publication Details

Applicant:
Indian Institute of Technology (IIT) Patna 
Author:
A. Raj, M. Dixit, M. Doble, A. K. Sen 
Corresponding Authors:
A. K. Sen 
DOI #:
10.1039/c7lc00599g 
Title:
A combined experimental and theoretical approach towards mechano-phenotyping of biological cells using a constricted microchannel 
Journal:
Lab on Chip 
Year:
2017 
Volume:
17 (21) 
Page:
3704–3716 
Keywords:
mechano-phenotyping, constricted microchannel 
Abstract:
We report a combined experimental and theoretical technique that enables the characterization of various mechanical properties of biological cells. The cells were infused into a microfluidic device that comprises multiple parallel micro-constrictions to eliminate device clogging and facilitate characterization of cells of different sizes and types on a single device. The extension ratio λ and transit velocity Uc of the cells were measured using high-speed and high-resolution imaging which were then used in a theoretical model to predict the Young’s modulus Ec = f(λ, Uc) of the cells. The predicted Young’s modulus Ec values for three different cell lines (182 ± 34.74 Pa for MDA MB 231, 360 ± 75 Pa for MCF 10A and, 763 ± 93 Pa for HeLa) compare well with those reported in the literature from micropipette measurements and atomic force microscopy measurement within 10% and 15%, respectively. Also, the Young’s modulus of MDA-MB-231 cells treated with 50 Μm 4-hyrdroxyacetophenone (for localization of myosin II) for 30 min was found out to be 260 ± 52 Pa. The entry time te of cells into the micro-constrictions was predicted using the model and validated using experimentally measured data. The entry and transit behaviors of cells in the microconstriction including cell deformation (extension ratio λ) and velocity Uc were experimentally measured and used to predict various cell properties such as the Young’s modulus, cytoplasmic viscosity and induced hydrodynamic resistance of different types of cells. The proposed combined experimental and theoretical approach leads to a new paradigm for mechanophenotyping of biological cells.  
Entered by:
Physics Head on 2020-08-04 
 
THE VISION
THE MISSION
ABOUT I-STEM
It has always been the basic tenet of the Government of India, in generously funding R&D efforts at academic institutions over the years, that facilities established through such support be made available to those needing them and qualified to make use of them for their own research work
read more >>

However, this was never easy or straightforward for, among other reasons, there was no ready source of information of what facility was available and where. Thanks to the Web, it is much easier today to have a national and regional “inventory of resources”, so as to match users with the resources they need, and to do all this in an efficient and transparent manner.

This can lead to a leap in R&D productivity and greatly enhance the effectiveness of public investment. This is the motivation behind I-STEM.
read less <<
Visitor Hit Counter
Hosted at Indian Institute of Science
Copyright © 2024 I-STEM. All rights reserved.
Audited by: STQC Bengaluru.