Indian Science Technology and Engineering facilities Map
 
Supplier Map
Service Map

Publications

Publication Details

Applicant:
Central University of Tamil Nadu 
Author:
Cui-Zan Cai 1 , Chuanbin Yang 2 , Xu-Xu Zhuang 1 , Ning-Ning Yuan 1 , Ming-Yue Wu 1 , Jie-Qiong Tan 3 , Ju-Xian Song 2 4 , King-Ho Cheung 2 , Huanxing Su 1 , Yi-Tao Wang 1 , Bei-Sha Tang 5 , Christian Behrends 6 , Siva Sundara Kumar Durairajan 2 7 , Zhenyu Yue 8 , Min Li 2 , Jia-Hong Lu 1 
Corresponding Authors:
Min Li, Jia-Hong Lu  
DOI #:
10.1080/15548627.2020.1760623 
Title:
NRBF2 is a RAB7 effector required for autophagosome maturation and mediates the association of APP-CTFs with active form of RAB7 for degradation 
Journal:
Autophagy 
Year:
2020 
Volume:
NA 
Page:
Na 
Keywords:
Autophagy, CCZ1-MON1A, maturation, NRBF2, PI3KC3/VPS34, RAB7, trafficking 
Abstract:
NRBF2 is a component of the class III phosphatidylinositol 3-kinase (PtdIns3K) complex. Our previous study has revealed its role in regulating ATG14-associated PtdIns3K activity for autophagosome initiation. In this study, we revealed an unknown mechanism by which NRBF2 modulates autophagosome maturation and APP-C-terminal fragment (CTF) degradation. Our data showed that NRBF2 localized at autolysosomes, and loss of NRBF2 impaired autophagosome maturation. Mechanistically, NRBF2 colocalizes with RAB7 and is required for generation of GTP-bound RAB7 by interacting with RAB7 GEF CCZ1-MON1A and maintaining the GEF activity. Specifically, NRBF2 regulates CCZ1-MON1A interaction with PI3KC3/VPS34 and CCZ1-associated PI3KC3 kinase activity, which are required for CCZ1-MON1A GEF activity. Finally, we showed that NRBF2 is involved in APP-CTF degradation and amyloid beta peptide production by maintaining the interaction between APP and the CCZ1-MON1A-RAB7 module to facilitate the maturation of APP-containing vesicles. Overall, our study revealed a pivotal role of NRBF2 as a new RAB7 effector in modulating autophagosome maturation, providing insight into the molecular mechanism of NRBF2-PtdIns3K in regulating RAB7 activity for macroautophagy/autophagy maturation and Alzheimer disease-associated protein degradation. 
Entered by:
Siva Sundara Kumar Durairajan on 2020-09-04 
 
THE VISION
THE MISSION
ABOUT I-STEM
It has always been the basic tenet of the Government of India, in generously funding R&D efforts at academic institutions over the years, that facilities established through such support be made available to those needing them and qualified to make use of them for their own research work
read more >>

However, this was never easy or straightforward for, among other reasons, there was no ready source of information of what facility was available and where. Thanks to the Web, it is much easier today to have a national and regional “inventory of resources”, so as to match users with the resources they need, and to do all this in an efficient and transparent manner.

This can lead to a leap in R&D productivity and greatly enhance the effectiveness of public investment. This is the motivation behind I-STEM.
read less <<
Visitor Hit Counter
Hosted at Indian Institute of Science
Copyright © 2024 I-STEM. All rights reserved.
Audited by: STQC Bengaluru.