Equipment Info

Mutation Detection System

Equipment Info

Equipment Code : 3311891

Make : VS20WAVE-DGGE,CSL-STIR, MU-DO 1

Model : VS20 Wave-DGGETC

Institution : Periyar University Salem

Department : Dept of Microbiology

Funding Agency Details : 0

Pooling of Equipment slot :   No

AMC End Date (Valid Till) :  

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PRISM Service Category (About PRISM) : D

Pre-Experiment Support (including sample preparation, setup assistance and pre-experimental guidance) :

Post-Experiment Support (including result interpretation, data analysis and post-experimental reporting) :

Equipment Certification (if any) (No document available):

Equipment Calibration (if any) (No document available):

Remarks / Special Instructions (if any):

Description : DGGE employs a polyacrylamide gel containing an increasing gradient of chemical denaturants (usually urea and formamide) through which the DNA molecules pass by electrophoresis. As double-stranded DNA passes through the gradient, each molecule will begin to denature at a particular concentration of denaturant dependent upon its %GC content and the exact arrangement of bases in the sequence. Denaturing molecules are retarded at the point of denaturation due to their newly acquired branched structure and can be visualized through electrophoresis, therefore achieving separation of molecules based on %GC content and DNA sequence rather than by size.

Usage Rate: (as indicated by the custodian/host institution)


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