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Equipment Info

High Performance Liquid Chromatography (HPLC)

Equipment Info

Equipment Code : 3311892

Make : SHIMADZU Preparative HPLC System

Model : LC-20AP

Institution : Periyar University Salem

Department : Dept of Microbiology

Funding Agency Details : DST, Govt. of India

Pooling of Equipment slot :   No

AMC End Date (Valid Till) :  

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PRISM Service Category (About PRISM) : D

Pre-Experiment Support (including sample preparation, setup assistance and pre-experimental guidance) :

Post-Experiment Support (including result interpretation, data analysis and post-experimental reporting) :

Equipment Certification (if any) (No document available):

Equipment Calibration (if any) (No document available):

Remarks / Special Instructions (if any):

Description : The High-performance liquid chromatography (analytical & preparative) was installed in the Department of Microbiology, Periyar University, Salem-11. High-performance liquid chromatography or commonly known as HPLC, is an analytical technique used to separate, identify or quantify each component in a mixture. The HPLC (Shimizu Japan) is equipped with photodiode array detector and florescent detector for analysis and separation of organic molecules. The preparative (C18, 10µm, 20 ×250mm) and analytical (C18, 5µm, 4.6 ×250mm) column used for HPLC analysis. The column packing is non-polar; the mobile phase is water+ miscible solvent (e.g Methanol and acetonitrile). It can be used for polar, non-polar, ignitable, and ionic samples. The total solvent flow rate is 1.0 ml/min analytical and 10.0 ml/min preparative. The column temperature will be maintained at 40? and pressure is set as maximum 449mpa. An injector is placed next to the pump; the total injection volume is 20 µL for analytical an

Usage Rate: (as indicated by the custodian/host institution)


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