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Equipment Info

Orbitrap High Resolution Liquid Chromatograph Mass Spectrometer (HRLCMS)

Equipment Info

Equipment Code : 2736837

Make : Thermo Fisher Scientific Pte. Ltd.

Model : Q-Exactive Plus Biopharma-High Resolution Orbitrap

Institution : Sophisticated Analytical Instrument Facilities (SAIF) IIT Bombay

Department : SAIF

Funding Agency Details : DST, Govt. of India

Pooling of Equipment slot :   No

AMC End Date (Valid Till) :  

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PRISM Service Category (About PRISM) : D

Pre-Experiment Support (including sample preparation, setup assistance and pre-experimental guidance) :

Post-Experiment Support (including result interpretation, data analysis and post-experimental reporting) :

Equipment Certification (if any) (No document available):

Equipment Calibration (if any) (No document available):

Remarks / Special Instructions (if any):

Description : The Orbitrap is an ion trap mass analyzer that consists of two outer electrodes and a central electrode, which enable it to act as both an analyzer and detector. Ions entering the Orbitrap are captured through "electrodynamic squeezing," after which they oscillate around the central electrode and in between the two outer electrodes. Different ions oscillate at different frequencies, resulting in their separation. By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection. Liquid chromatography / Mass Spectroscopy (LC / MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectroscopy,a powerful analysis & detection technique. There are two common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI) & Atmospheric Pressure Chemical Ionization (APCI). Both are soft ionization technique.

Usage Rate: (as indicated by the custodian/host institution)


Samples External Academic User Internal Academic User Industry User Start-ups National Research Lab User Grassroot innovator
Only HRMS
HRMS + compound formula & library search
LCMS with library (Plant extract or Impurity Profiling or Metabolite Identification or Degradation studies or Environmental samples)
Protein Identification (for 1-hour)
Protein Identification (for 2-hours)
Sample Preparation (In-gel or In-solution Trypsin Digestion with Desalting)
Only desalting of digested protein samples
Lipidomics
Intact Mass of Proteins Peptides and Oligonucleotides

Accessories / Consumables / Spare parts / Testing modes

Sample Name Sample Type Sample UOM Sample Availability Unit Cost Sample Quantity Sample Description
No Data Available

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