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Equipment Info

Confocal Laser Scanning Microscope (CLSM)

Equipment Info

Equipment Code : 38938

Make : Carl Zeiss, Germany,

Model : LSM 510

Institution : CSIR Central Scientific Instruments Organisation (CSIO) Chandigarh

Department : Ubiquitous Analytical Techniques

Funding Agency Details : DST, Govt. of India

Pooling of Equipment slot :   No

AMC End Date (Valid Till) :  

PRISM Service Category (About PRISM) : D

Pre-Experiment Support (including sample preparation, setup assistance and pre-experimental guidance) :

Post-Experiment Support (including result interpretation, data analysis and post-experimental reporting) :

Equipment Certification (if any) (No document available):

Equipment Calibration (if any) (No document available):

Remarks / Special Instructions (if any):

Description : The design of a confocal microscope is very similar to a conventional epi-fluorescence microscope except with additional inclusion of confocal optics. The confocal optics consists of a confocal aperture (adjustable pinholes) which is such placed that it is conjugates the image plane (i.e., focal plane) of the objective lens. The diameter of confocal aperture is infinitesimally small, thus PMT detector can only detect light passing through the pinhole. This implies that, light originating in the focal plane of objective lens can only reach to detector. The very small confocal aperture also allows point detection of the sample. As a result, laser are used as excitation source in confocal and image is captured by point-by-point detection across the whole sample (i.e., raster imaging). Due to exclusion of light from non-focal planes and point detection, highly contrast images are generated in confocal microscopes. Another important aspect of CLSM system is optical sectioning. Due to

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